RESUMO
Coronavirus disease (COVID-19) is an acute infectious disease of the respiratory tract caused by a new SARS-CoV-2 coronavirus. A global vaccination program against SARS-CoV-2 continues, and the incidence of COVID-19 worldwide is significantly decreasing. However, among millions of those who survived COVID-19, numerous groups will need assistance due to increased clinical consequences after COVID-19. Currently, there is a need to search for molecular biomarkers for monitoring the onset and progression of post-COVID syndrome. For this purpose, the relative average length of chromosome regions was studied in the groups of women of reproductive age: in the group of patients (n = 64) recovered from COVID-19 and in the control group (n = 42) of women of the same age. The analysis was carried out using a method of multiplex monochrome quantitative real-time PCR on DNA samples isolated from the peripheral blood leukocytes. According to the results of the study, it was established that the relative average length of chromosomes in the peripheral blood leukocytes was statistically significantly lower in the group of patients with COVID-19 than in the control group (p < 0.05). The results obtained allow one to state that the observed shortening of the relative average length of telomeres in the group of patients that recovered from COVID-19 can indicate that SARS-CoV-2 infection can directly cause the erosion of telomeres in the blood cells, particularly, in leukocytes. Thus, the determination of the relative average length of telomeres can be an informative prognostic marker for estimating the risk of the severity of COVID-19 disease and the development of post-COVID syndrome.
RESUMO
OBJECTIVE: analysis of some reproductive health disorders of female residents of radioactively contaminated (con- taminated) and clean territories of Ukraine. MATERIALS AND METHODS: Data on disorders of the menstrual cycle (DMC), cancer of the body of the uterus (BU), inflammatory diseases of the uterus cervix (IDUC), which are taken from the statistical reports of the Ministry of Healthcare of Ukraine, from the State Statistics Service of Ukraine and the Kiev Regional Center for Maternal and Child Health Depending on Residence for the period 2007-2017, depending on the residence in the radioactively contaminated or clean settlement. RESULTS: The prevalence and incidence of DMС increased from 2007 to 2017 from 17.79 and 10.66 to 18.50 and 11.66 , the prevalence and incidence rates of IDUC (from 10.05 and 7.11 to 12.50 and 8.60 ), there was a negative tendency of incidence of malignant neoplasms of BU (an increase of 32.2 %) and UC (an increase of 5.1%). The incidence of BU cancer in 2014-2017 compared to Ukraine as a whole (0.14 ) in Kyiv region and Zhytomyr region increased (0.17 , p <0.01 and 0.17 , p < 0.01), and in Rivne region it was no diffe- rent from the national one (0.12 ). The lowest prevalence and incidence of DMC were found in Rivne region (10.41 and 6.85 ), which is almost twice lower than in Zhytomyr region (24.78 and 11.16 ). In Kyiv region, these figures are also significantly lower than in Ukraine (14.48 and 10.10 ). In the Kyiv region, there was no difference in the frequency of DMC (389.57 , versus 405.99 , p = 0.72), hyperplasia (67.48 vs. 59.95 , p = 0.80), and BU polyps (46.01 against (57.22 , p = 0.62) in contaminated and clean territory, with no such difference found for women from 1970-1987. CONCLUSIONS: Against the background of an increased incidence of BU cancer in the contaminated territories of the Zhytomyr region and Kyiv region, the prevalence and incidence of DMC was higher than the national level only in Zhytomyr region, whereas in Kyiv region and Rivne region, on the contrary, the indicators were lower. There is no dif- ference in the frequency of DMC, endometrial hyperplasia, BU polyps, both individually and in combination, among women who lived in the contaminated and clean territory of the Kyiv region.
